POS0742 SCREENING AND BIOINFORMATICS ANALYSIS OF HUB GENES AND PATHWAYS FOR PRIMARY SJÖGREN’S SYNDROME BASED ON GEO DATABASE

Background: Primary Sjögren’s syndrome (pSS) is an autoimmune disease that featured as lymphoplasmacytic infiltration of the exocrine glands leading to sicca symptoms 1 . However, its underlying molecular mechanisms remain elusive. Objectives: This study aims identify differentially expressed genes (DEGs) and pathways associated with progression pSS using bioinformatics analysis explore pathogenesis. Methods: The pSS-associated gene chip data set GSE66795 was obtained from Gene Expression Omnibus (GEO) database, which included 131 cases fully-phenotyped patients’ whole blood samples 29 control samples. DEGs were screened Using R software. Online tool Metascape 2 used make Ontology (GO) KEGG pathway enrichment. PPI network performed String database. Hub identified by Cytoscape. Results: A total 108 captured, including 101 up-regulated 7 down-regulated genes. GO enrichment showed these primarily enriched in defense response virus, interferon-gamma, regulation innate immune response, interferon-beta, double-stranded RNA binding, interferon-alpha. principally Influenza A, RIG-I-like receptor signaling pathway, necroptosis, Staphylococcus aureus infection. Finally, 9 hub (STAT1, IRF7, OAS2, GBP1, OAS1, IFIT3, IFIH1, OAS3, DDX60) had highest degree value. Conclusion: findings key may involve occurrence development pSS. References: [1]Francois H, Mariette X. Renal involvement primary Sjogren syndrome. Nat Rev Nephrol 2016;12(2):82-93. doi: 10.1038/nrneph.2015.174 [published First: 2015/11/17]. [2]Zhou Y, Zhou B, Pache L, et al. provides a biologist-oriented resource for systems-level datasets. Commun 2019;10(1):1523. 10.1038/s41467-019-09234-6 2019/04/05]. Acknowledgements: project supported National Science Foundation China (82001740), Open Fund Key Laboratory Cellular Physiology (Shanxi Medical University) (KLCP2019) Innovation Plan Postgraduate Education Shanxi Province (2020BY078). Disclosure Interests: None declared